negative staining การใช้
- Liposomes can be easily distinguished from micelles and hexagonal lipid phases by negative staining transmission electron microscopy.
- Furthermore, negative staining with nigrosin can reveal some microorganisms that cannot be stained by regular methods.
- For bright field microscopy, negative staining is typically performed using a black ink fluid such as nigrosin.
- Sample preparations involve depositing specimens onto a coated TEM grid and negative staining with an electron-opaque liquid.
- The virus is about 28 nm in size, and can be observed with transmission electron microscopy using negative staining.
- In biology, nigrosin is used for negative Staining of bacteria ., as well as the capsule-containing fungus, " Cryptococcus neoformans ".
- The "'Synergistetes "'is a recently recognized phylum of anaerobic bacteria that show Gram-negative staining and have rod / vibrioid cell shape.
- The elastic lamina is best visualized using Verhoeff's stain, but can be easily detected in specimens stained using other techniques as a well-defined negative staining region.
- In the biological sciences in order to withstand the instrument vacuum and facilitate handling, biological specimens can be fixated using either a negative staining material such as uranyl acetate or by plastic embedding.
- Size and shapes of lamellar, micellar and hexagonal phases of lipid bilayer phase behavior and mixed lipid polymorphism in aqueous dispersions can be easily identified and characterized by negative staining transmission electron microscopy too.
- The complex structure of the V-ATPase has been revealed through the structure of the " M . Sexta " and Yeast complexes that were solved by single-particle cryo-EM and negative staining, respectively ( Muench 2009, Diepholz 2008, Zhang 2008 ).